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In vivo Porphyrin Fluorescence from <i>Propionibacterium acnes</i>. A Characterization of the Fluorescing Pigments
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1982
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EngineeringPorphyrin MetabolismPhotobiologyMolecular BiologyBioanalysisNatural FluorescenceAnalytical ChemistryPhotophysical PropertyMolecular ImagingBiophysicsPhotochemistryBiochemistryFluorescence MicroscopyVivo Porphyrin FluorescenceMetal-free CoproporphyrinMicrobiologyFluorescing PigmentsMedicinePigment
The natural fluorescence from <i>Propionibacterium acnes</i> has been investigated in vivo. The fluorescence spectrum can be decomposed into three distinct emission spectra due to the presence of metal-free coproporphyrin, protoporphyrin and uroporphyrin in the bacteria. The amounts of these substances can be estimated from the in vivo fluorescence measurements, which may serve as a nondestructive technique to study the porphyrin metabolism.