Canadian Journal of Biochemistry · 1966 · 55 citations · 0 references
Life SciencesCell BiologyPhysiologyAnimal PhysiologyCell PhysiologyCellular MetabolismDigestive PhysiologyEpithelial TissueGastrointestinal FunctionGastrointestinal PhysiologyIntestinal AbsorptionIntestinal Barrier FunctionEnteroendocrine CellsSmall Intestinal Function
A new method for the preparation of isolated intestinal epithelial cells from the rat is described. Suspensions of these cells respire actively, taking up oxygen in a linear relationship with time for about 40 minutes. When actively transported sugars are present in the incubation medium, the cells utilize more oxygen. It is suggested that such preparations may be useful in the study of intestinal function at the cellular level.