Publication | Closed Access
Evolution of Herpes Simplex Cellular Lesions Observed in vitro by Phase Contrast Microcinematography
34
Citations
0
References
1959
Year
Phase Contrast MicrocinematographyDiagnostic VirologyCell DivisionMicroscopyProgressive DissolutionCell Division MechanismHistopathologyHerpes Simplex VirusPathologyVirologyHerpesvirusesCell CultureDermatologyMedicineCell Biology
Continuous phase contrast observation and microcinematographic recording of living rabbit kidney cell cultures infected with herpes simplex virus, established the following phenomena: 1. Progressive dissolution and dispersion of nucleoli, loss of nucleo-plasmic homogeneity, and appearance of intranuclear densification zone but not of a well delineated inclusion body. 2. Persistent activity of cytoplasmic organelles in cells with severe nuclear lesions. 3. Rapid (2-3 hours) formation of giant cells by cell confluence but not by cell division mechanism, accompanied by signs of cytoplasmic liquefaction. The significance of these data is discussed.