Concepedia

Facile Activation of Sepharose Hydroxyl Groups by 2-Fluoro-1 Methylpyridinium Toluene-4-Sulfonate: Preparation of Affinity and Covalent Chromatographic Matrices

T. T. Ngo

Nature Biotechnology · 1986 · 53 citations · 13 references

DOI

Concepts

References

13

Tissue sulfhydryl groups

George L. Ellman · Archives of Biochemistry and Biophysics · 1959 · 26.2K citations

New Synthetic Reactions Based on the Onium Salts of Aza‐Arenes [New synthetic methods (29)]

Teruaki Mukaiyama · Angewandte Chemie International Edition in English · 1979 · 316 citations

, , +9

Immobilization of enzymes and affinity ligands to various hydroxyl group carrying supports using highly reactive sulfonyl chlorides

Kurt Nilsson, Klaus Mosbach · Biochemical and Biophysical Research Communications · 1981 · 233 citations

, , +16

A new approach (cyano-transfer) for cyanogen bromide activation of Sepharose at neutral pH, which yields activated resins, free of interfering nitrogen derivatives

Joachim Kohn, Meir Wilchek · Biochemical and Biophysical Research Communications · 1982 · 212 citations

, , +5

[3] Immobilization of enzymes to agar, agarose, and sephadex support

Jerker Porath, Rolf Axén · Methods in enzymology on CD-ROM/Methods in enzymology · 1976 · 150 citations

, , +12