FEBS Letters · 2004 · 49 citations · 24 references
Life SciencesGeneticsMolecular BiologyGene ExpressionGene RegulationTranscriptional RegulationOxidative StressPathway RegulationRedox SignalingStress Response GenesStress Signaling PathwaysStress-responsive Gene RegulationCellular Oxidative Stress ResponseCellular Response To Oxidative StressOxidative Stress RegulationOxidative Stress Response PathwaysOxidative Stress Signaling PathwaysPromoter-proximal ElementsPromoter-proximal RegulationProximal Promoter Elements
The GPX2 gene encodes a homologue of phospholipid hydroperoxide glutathione peroxidase in Saccharomyces cerevisiae. The GPX2 promoter contains three elements the sequence of which is completely consistent with the optimal sequence for the Yap1 response element (YRE). Here, we identify the intrinsic YRE that functions in the oxidative stress response of GPX2. In addition, we discovered a cis-acting element (5'-GGCCGGC-3') within the GPX2 promoter proximal to the functional YRE that is necessary for H(2)O(2)-induced expression of GPX2. We present evidence showing that Skn7 is necessary for the oxidative stress response of GPX2 and is able to bind to this sequence. We determine the optimal sequence for Skn7 to regulate GPX2 under conditions of oxidative stress to be 5'-GGC(C/T)GGC-3', and we designate this sequence the oxidative stress-responsive Skn7 response element.
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A Thiol Peroxidase Is an H2O2 Receptor and Redox-Transducer in Gene Activation
A Delaunay, Delphine Pflieger, Marie-Bénédicte Barrault et al. · Cell · 2002 · 907 citations · Full text