Journal of Food Biochemistry · 1980 · 186 citations · 12 references
Food ChemistryEngineeringBiochemistryMedicineBioanalysisGlycobiologyPotato CultivarsPolysaccharideSeed StorageProtein PurificationPotato TubersCarbohydrate-protein InteractionBiomolecular EngineeringChromatographyMajor Soluble GlycoproteinGlycosylation
The major soluble glycoprotein of potato tubers was isolated by chromatography on DEAE cellulose and Con A Sepharose. It contained about 5% neutral sugar and 1%hexosamine, exhibited a molecular weight of 45,000 by SDS gel electrophoresis, and consisted of 6–10 ionic forms as revealed by isoelectric focusing. This glycoprotein comprised about 20%of the soluble protein in all of the 31 tested potato cultivars.
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