Twenty‐three neutrophil granulocytes in 10 high‐power fields is the best histopathological threshold to differentiate between aseptic and septic endoprosthesis loosening

Abstract

We therefore recommend a counting algorithm with a threshold of > or =23 NG in 10 HPF (visual field diameter 0.625 mm) for the histopathological diagnosis of septic endoprosthesis loosening. If the enumeration of NG is difficult in conventional haematoxylin and eosin-stained slides, CD15 immunohistochemistry should be performed, whereas the PAS stain has not proven to be helpful.