Pyrene Excimer‐Based Dual‐Emission Detection of a Oligoaspartate Tag‐Fused Protein by Using a Zn^II–DpaTyr Probe

Abstract

A dual-emission sensing system for proteins tethered to a repeated tetra-aspartate tag (D4-tag) was successfully developed by using the binding-induced excimer formation of the pyrene-appended ZnII–DpaTyr probe. This allows selective detection of the D4-tag-fused protein in a simple and convenient manner by the naked eye. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2268/2007/z700146_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.