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On the Mechanism of Adenyl Cyclase Inhibition by Adenosine
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1973
Year
Cellular PharmacologyCyclic Amp PathwayExperimental PharmacologyCellular PhysiologyMolecular PharmacologyAdenyl Cyclase InhibitionInhibitory ActivityMolecular PhysiologyBiochemistryMechanism Of ActionEndocrinologyPharmacologyMm AdenosineBasal ActivitySignal TransductionNatural SciencesPhysiologyCellular BiochemistryMetabolismMedicine
Adenosine inhibited basal and fluoride- or hormone-stimulated adenyl cyclase activities from various sources. Inhibition was usually noted at 0.01 mM adenosine and was 40–75% at 1 mM. The effect appeared to be rapid and reversible, but it was not competitive with respect to ATP, as determined with enzyme from rat brain. Stimulated adenyl cyclase activities from rat fat cells and Ehrlich ascites cells were proportionately more inhibited than basal activity indicating the possibility of multiple sites for inhibitory action by adenosine. The effect of adenosine on adenyl cyclase and other enzymes of the cyclic AMP pathway may be of pharmacological or physiological significance in certain tissues.