Sites of synthesis and transport of photosynthetic products within the leaf cell

Abstract

1. After illumination of leaves in the presence of 14CO2 for various times and subsequent freeze drying, chloroplasts were isolated using a nonaqueous procedure. The time-course of the distribution of a number of compounds between chloroplasts and the remainder of the cell was calculated from the 14C-incorporation into the fractions obtained. 2. Labelled ribulose diphosphate, sedoheptulose diphosphate and sedoheptulose monophosphate occurred, at least during the first minutes of photosynthesis, solely in the chloroplasts. At the beginning of photosynthesis phosphoglyceric acid, fructose diphosphate, fructose monophosphate and glucose monophosphate appeared first in the chloroplasts, but were found later also in the non-chloroplastic part of the cell. The major part of glucose diphosphate, uridine diphosphoglucose, sucrose, malic acid and citric acid was always located in the non-chloroplastic part of the cell. 3. From the results it is concluded that the photosynthetic carbon cycle operates exclusively in the chloroplasts. Sugar phosphates, which are not needed in the cyclic regeneration of the CO2-acceptor, are directly translocated into the cytoplasm. The synthesis of uridine diphosphoglucose takes place mainly in the cytoplasm. Glucose diphosphate and possibly also sucrose seem to be formed in the cytoplasm of the leaf cell.