Direct Electrochemistry of Hemoglobin and Its Electrocatalysis Based on Hyaluronic Acid and Room Temperature Ionic Liquid

Abstract

Abstract A biocomposite material of hyaluronic acid (HA) and room temperature ionic liquid, 1‐butyl‐3‐methyl‐imidazolium tetrafluoroborate (BMIMBF 4 ) was fabricated and characterized by voltammetry and electrochemical impedance spectroscopy. Hemoglobin (Hb), as a model protein, was immobilized in this biocomposite on glassy carbon electrode to investigate its direct electrochemistry. A pair of well‐defined, quasireversible cyclic voltammetric peaks appeared with the formal potentials ( E °′) at −0.360 V (vs. SCE). The Hb modified electrode exhibited an excellent electrocatalytic activity to the reduction of H 2 O 2 with a wide linear range, good selectivity, sensitivity and reproducibility, which had potential application to fabrication the third‐generation biosensor.