Formation of Giant Lipid Vesiclelike Compartments from a Planar Lipid Membrane by a Pulsed Jet Flow

Abstract

We report a straightforward method for the preparation of lipid vesicles inspired by forming soap bubbles from a soap film. Vesicles are mechanically blown out of a preformed biofunctional planar lipid membrane by applying a short pulsed liquid jet flow ejected from a fine jet nozzle brought near the membrane. This method allows us to directly encapsulate any molecules or substances of interest into the uniformly sized vesicles in a short time.