Adaptation of an Insect Cell Line (Grace's Antheraea Cells) to Medium Free of Insect Hemolymph

Abstract

Cultures of the insect cell line derived by Grace from Antheraea eucalypti Scott [Austrocaligula eucalypti (Scott)] were successfully adapted to medium supplemented with fetal bovine serum, whole-egg ultrafiltrate, and bovine plasma albumin instead of insect hemolymph. Cells, now in their 37th passage, have a population doubling time of 2.5 days; those of unadapted cultures, 4.2 days.