In CHEF Electrophoresis a Linear Induction of Dsb Corresponds to a Nonlinear Fraction of Extracted DNA with Dose

Abstract

A theory for DNA double-strand break (dsb) analysis by pulsed field electrophoresis is presented, based on the size distribution of DNA fragments, which converts an induction of dsb linear with dose into a nonlinear fraction of extractable DNA, in agreement with experimental observations.