The Quantitative Measurement of DNA Hybridization from Renaturation Rates

TLDR

The study proposes a new method to quantify bacterial relatedness by measuring DNA renaturation rates of individual types and their mixtures. Equations linking binding degree to renaturation rates were derived for multiple scenarios. Optimal measurement conditions were established, the method was validated in several tests, and its advantages were summarized.

Abstract

A new method is proposed to measure relatedness amongst bacteria, based on renaturation rate determinations of DNA types and their mixture. The equations, relating the degree of binding with renaturation rates, are calculated for several cases. The optimal conditions for measurements are given. The method was checked in several ways. The advantages are summarized.