Selective Enzyme-Linked Immunosorbent Assay for Triclosan. Application to Wastewater Treatment Plant Effluents

Abstract

A sensitive and highly selective enzyme-linked immunosorbent assay was developed for triclosan, one of the most common bactericides in personal care products, which is also considered as an emerging contaminant, given its presence in the effluents of sewage and wastewater treatment plants. From synthesized haptens, polyclonal rabbit antibodies against triclosan were raised. The best ELISA immunoassay was based on an antibody-coated format, yielding a detection limit of 0.03 microg L(-1), an I50 of 3.85 microg L(-1), and a dynamic range from 0.22 to 42.16 microg L(-1), with little or no cross-reactivity (< 10%) to similarly structured compounds, including its metabolite methyltriclosan (CR < 6%). The assay was applied as a screening method to quantify triclosan in surface water and in wastewaters. After C18 solid-phase extraction, nanogram per liter concentrations were determined in effluents of urban wastewater treatment plants. The satisfactory recoveries achieved as well as the agreement between immunochemical and chromatographic methods (GC-MS) indicate its potential for either screening or laboratory quantification.