Genome walking by single-specific-primer polymerase chain reaction: SSP-PCR

[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction

Kary B. Mullis, Fred Faloona · Methods in enzymology on CD-ROM/Methods in enzymology · 1987 · 5.7K citations

Life Sciences, Genetics, Molecular Biology +11

Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

Michael A. Frohman, Michael Dush, Gail R. Martin · Proceedings of the National Academy of Sciences · 1988 · 4.6K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +13

Analysis of enzymatically amplified β-globin and HLA-DQα DNA with allele-specific oligonucleotide probes

Randall K. Saiki, Teodorica L. Bugawan, Glenn T. Horn et al. · Nature · 1986 · 1.8K citations

Life Sciences, Genetics, Molecular Genetics +14

The physical map of the whole E. coli chromosome: Application of a new strategy for rapid analysis and sorting of a large genomic library

Yuji Kohara, Kiyotaka Akiyama, Katsumi Isono · Cell · 1987 · 1.8K citations

Life Sciences, Genetics, Microbiology +15

A procedure for<i>in vitro</i>amplification of DNA segments that lie outside the boundaries of known sequences

Tony Triglia, M G Peterson, David J. Kemp · Nucleic Acids Research · 1988 · 812 citations · Full text

Life Sciences, Genetics, Molecular Biology +9