Chlorophyll <i>a</i> and β‐carotene content in the D₁/D₂/cytochrome <i>b</i>‐559 reaction center complex from spinach

Abstract

Pigment stoichiometries in the D 1 /D 2 /cytochrome b ‐559 reaction center complex from spinach have been studied by spectrophotometry and normalphase silica HPLC of the solvent extracts. Based on the well‐accepted molar ratio Pheo a /P680 = 2, the results are summarized by Chl a /P680 = 6.01 ± 0.37 ( n = 24) and β‐carotene/P680= 1.84 ± 0.11 ( n = 4). These stoichiometries are significantly different from those of bacterial reaction center complexes.