FEBS Letters · 2000 · 15 citations · 26 references
Life SciencesCell BiologyMolecular BiologyCell SignalingElectrophysiologyIon Channel BiophysicsCalcium SignalingCalcium Transport ProteinsIntracellular Calcium SignalingMuscle PhysiologyMuscle TissueNeuromuscular JunctionCalcium Channel CouplingCalcium Channel PhysiologyCalcium Ion SignalingCalcium Signaling In Muscle CellsCurrentsExcitation-contraction CouplingGreen Fluorescent Protein ReportersSkeletal Muscle Physiology
A full-length and a C-terminally truncated form of the calcium channel alpha(1S) subunit can be isolated from skeletal muscle. Here we studied whether full-length alpha(1S) is functionally incorporated into the skeletal muscle excitation-contraction coupling apparatus. A fusion protein of alpha(1S) with the green fluorescent protein attached to its C-terminus (alpha(1S)-GFP) or alpha(1S) and GFP separately (alpha(1S)+GFP) were expressed in dysgenic myotubes, which lack endogenous alpha(1S). Full-length alpha(1S)-GFP was targeted into triad junctions and restored calcium currents and excitation-contraction coupling. GFP remained colocalized with alpha(1S), indicating that intact alpha(1S)-GFP was inserted into triads and that the C-terminus remained associated with the excitation-contraction coupling apparatus.
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Primary structure and functional expression from complementary DNA of a brain calcium channel
Yasuo Mori, Thomas Friedrich, Man-Suk Kim et al. · Nature · 1991 · 869 citations