Spirostanol Saponins from the Fibrous Roots of <i>Ophiopogon japonicus</i> (<scp>Thunb</scp>.) <scp>Ker‐Gawl</scp>

Abstract

Abstract Three new steroidal saponins, (25 R )‐ruscogenin‐3‐yl α ‐ L ‐rhamnopyranosyl‐(1→2)‐[ β ‐ D ‐xylopyranosyl‐(1→4)]‐ β ‐ D ‐glucopyranoside ( 1 ), diosgenin‐3‐yl 2‐ O ‐acetyl‐ α ‐ L ‐rhamnopyranosyl‐(1→2)‐[ β ‐ D ‐xylopyranosyl‐(1→4)]‐ β ‐ D ‐glucopyranoside ( 2 ), and pennogenin‐3‐yl 2‐ O ‐acetyl‐ α ‐ L ‐rhamnopyranosyl‐(1→2)‐[ β ‐ D ‐xylopyranosyl‐(1→4)]‐ β ‐ D ‐glucopyranoside ( 3 ) were isolated from the fibrous roots of Ophiopogon japonicus ( Thunb .) Ker‐Gawl . Their structures were determined by spectroscopic methods including IR, HR‐ESI‐MS, and 1D‐ and 2D‐NMR. All of these three steroidal saponins exhibited weak cytotoxicities against Hela and Hep2 cell lines.