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Cloning of the Creatinine Amidohydrolase Gene from<i>Pseudomonas</i>sp. PS-7
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1995
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BiosynthesisEngineeringBiochemistryEnzyme CatalysisBiotechnologyPseudomonas SpCreatinine AmidohydrolaseStructure-function Enzyme KineticsMicrobiologyMolecular MicrobiologyEc 3.5.2.10Creatinine Amidohydrolase GeneMedicineEnzymatic ModificationProtein BiosynthesisMicrobial Genetics
The gene encoding creatinine amidohydrolase (EC 3.5.2.10) was isolated from Pseudomonas sp. PS-7. The primary structure of creatinine amidohydrolase deduced from the nucleotide sequence showed that the protein is composed of 259 amino acids and has a molecular weight of 28,437. The enzymatic property of creatinine amidohydrolase produced by recombinant E. coli was identical with those by Pseudomonas sp. PS-7.