Journal of General Virology · 2009 · 14 citations · 30 references
AgriculturePlant BiologyPlant SciencesMolecular VirologyPlant VirologyVirologyPlant Virus ReplicationPlant Virus ResistancePlant Virus SymptomatologyPlant VirusesViral Rna BiologyVirus-host InteractionsAssessment Of Symptom ExpressionCdna CloningFull-length Cdna SequencingPlant Viral PathogenesisViral Replicase ComplexVirus DiseasesVirus SurvivalWestern Blotting
For a better understanding of the functionality and pathogenicity of beet soil-borne virus (BSBV), full-length cDNA clones have been constructed for the three genomic RNAs. With the aim of assessing their effectiveness and relative contribution to the virus housekeeping functions, transcripts were inoculated on Chenopodium quinoa and Beta macrocarpa leaves using five genome combinations. Both RNAs-1 (putative replicase) and -3 (putative movement proteins) proved to be essential for virus replication in planta and symptom production on C. quinoa, whereas RNA-2 (putative coat protein, CP, and a read-through domain, RT) was not. No symptoms were recorded on B. macrocarpa, but viral RNAs were detected. In both host plants, the 19 kDa CP was detected by Western blotting as well as a 115 kDa protein corresponding to the CP-RT.
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Nucleotide Sequence of Beet Necrotic Yellow Vein Virus RNA-2
S. Bouzoubaa, V. Ziegler, David L. Beck et al. · Journal of General Virology · 1986 · 142 citations
Patrice Dunoyer, Sébastien Pfeffer, C. Fritsch et al. · The Plant Journal · 2002 · 141 citations · Full text