Polymerase chain reaction for detection of the pMP6d-9/MspI RFLP, a marker closely linked to the cystic fibrosis mutation

Abstract

SOURCE/DESCRIPTION: pMP6d-9 is a human genomic clone with a 2.6kb HindIll insert in pBR322(1). HindIII/Mspl subclones were sequenced in order to obtain oligonucleotides suitable for detection of the M8pI RFLP within pMP6d-9 by PCR. POLYMORPHISM: With Southern btotting the Mspt RFLP has alleles of 13kb(allele 1) and 8.5 + 4.5kb(allele 2). Allele 1 represents the absence