A New RNA Synthetic Method with a 2‘- <i>O</i> -(2-Cyanoethoxymethyl) Protecting Group

Abstract

A novel method for the synthesis of RNA oligomers with 2-cyanoethoxymethyl (CEM) as the 2'-hydroxyl protecting group has been developed. The new method allows the synthesis of oligoribonucleotides with an efficiency and final purity comparable to that obtained in DNA synthesis. [structure: see text]