Development and characterization of polymorphic microsatellites from <i>Prunus avium</i>‘Napoleon’

Abstract

Abstract Primers were developed for 21 microsatellite loci isolated by enrichment from Prunus avium ‘Napoleon’. Twelve loci contained uninterrupted dinucleotide repeats and nine were more complex. Nineteen primer pairs (EMPA001–019) showed single locus polymorphisms in a cultivar survey of 14 sweet cherries, with two to seven alleles per locus. Three primer pairs in combination (EMPA014, 015 and 018) discriminated all cultivars. Two primer pairs for loci monomorphic in P. avium were included: EMPA020 revealed segregation in an interspecific progeny and EMPA021 revealed polymorphism in P. dulcis . Twelve primer pairs reliably amplified products in three peach cultivars of which seven revealed polymorphisms.