<scp>DNA</scp>‐Dependent <scp>DNA</scp> Polymerases

Abstract

There are two types of DNA-dependent RNA polymerases commonly used for nucleic acid research: E. coli RNA polymerase and the bacteriophage T7, T3, and SP6 RNA polymerases. This unit describes the uses and limitations of each class of RNA polymerase along with specific reaction conditions and applications.