Subcellular Localization of Two Long‐Chain Acyl‐Coenzyme‐A Synthetases in <i>Candida lipolytica</i>

Abstract

Studies have been made on the subcellular localization of two long‐chain acyl‐coenzyme‐A synthetases as well as glycerolphosphate acyltransferase and the acyl‐CoA‐oxidizing system in Candida lipolytica grown on oleic acid. Acyl‐CoA synthetase I is distributed among different subcellular fractions, including microsomes and mitochondria where glycerolphosphate acyltransferase is located. On the other hand, acyl‐CoA synthetase II is localized in microbodies where the acyl‐CoA‐oxidizing system is located. These results support our previous conclusion that acyl‐CoA synthetase I is responsible for the production of acyl‐CoA to be utilized for the synthesis of cellular lipids, while acyl‐CoA synthetase II provides acyl‐CoA that is exclusively degraded via β‐oxidation.