Isolation of Erwinia amylovora Bacteriophage from Aerial Parts of Apple Trees

Abstract

RITCHIE, D. F., and E. J. KLOS. 1977. Isolation of Erwinia amylovora bacteriophage from aerial parts of apple trees. Phytopathology 67:101-104. Populations of Erwinia amylovora bacteriophage greater a spreading translucent halo and a smaller plaque without a than 106 plaque-forming units (PFU) per gram of tissue were halo. Thirty-five bacterial isolates, consisting of nine genera, isolated without enrichment from diseased aerial parts of 18 species, and 15 strains of E. amylovora were typed; the apple trees during the summer of 1975. Three phage isolates phages lysed only E. amylovora. The burst size of the three were selected from different geographical locations. Two isolates was 40 to 50 PFU per cell. The phages could be stored types of plaques were produced; a clear-centered plaque with at 4 C and -20 C but lost titer when stored at 24 C.