Research Note: The Evaluation of Chicken Spermatozoa Using Fluorescent Staining in a 96-Well Format

Abstract

An efficient method for the evaluation of spermatozoa using the fluorescent stains carboxyfluorescein diacetate and propidium iodide and an automated fluorescence concentration analyzer was adapted for chicken semen. Arbitrary fluorescence units representing either live or dead spermatozoa were strongly correlated with percentage of added dead spermatozoa and with the direct fluorescent microscope counts of live, dead, and damaged cells.