Nucleic Acids Research · 1983 · 84 citations · 39 references
Life SciencesBiologyMolecular BiologyGene StructureRna ProcessingStructural BiologyRibosomal Rna ProcessingRibosome BiogenesisRibosome StructureRna FoldingRna StructureTranscription In EukaryotesFolding PathwaysFolding PatternsNucleotide Sequence AlignmentRibosomal Rna FoldingRibosomal Rna TranscriptionRibosome Biogenesis In ProkaryotesRna Folding Pathways
We have determined the complete nucleotide sequence of the regions of mouse ribosomal RNA transcription unit which separate mature rRNA genes. These internal transcribed spacers (ITS) are excised from rRNA precursor during ribosome biosynthesis. ITS 1, between 18S and 5.8S rRNA genes, is 999 nucleotides long. ITS 2, between 5.8S and 28S rRNA genes, is 1089 nucleotides long. Both spacers are very rich in G + C, 70 and 74% respectively. Mouse sequences have been compared with the other available eukaryotes: while no homology is apparent with yeast or xenopus, mouse and rat ITS sequences have been largely conserved, with homologous segments interspersed with highly divergent tracts. Homology with rat is much more extensive for ITS 1 than for ITS 2. Tentative secondary structure models are proposed for the folding of these regions within rRNA precursor; they are closely related in mouse and rat.
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Improved Estimation of Secondary Structure in Ribonucleic Acids
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Molecular structure of a left-handed double helical DNA fragment at atomic resolution
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