Nucleic Acids Research · 1984 · 484 citations · 33 references
Life SciencesMolecular BiologyMolecular GeneticsGene ExpressionTranscriptionTranscriptional RegulationNicotinamide Adenine Dinucleotide MetabolismTranscriptomic AnalysisCdna CloningCdna Library ConstructionCdna SynthesisPost-transcriptional Gene RegulationPost-transcriptional RegulationTissue-specific Gene Expression
We have isolated and identified cDNA clones containing part of the coding sequence for rat glyceraldehyde-3-phosphate-dehydrogenase (GAPDH, E.C. 1.2.1.12). By using one of these clones as a probe, we have shown that: i) the abundance of GAPDH mRNA is different in various tissues of the adult rat and in good correlation with the abundance of the enzyme; ii) the transcription rates are quite similar in all tissues tested. We therefore conclude that the tissue-specific differential GAPDH gene expression is regulated by adjusting the abundance of its mRNA at the post-transcriptional level.
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Haider Raza, Annie John, Abderrahim Nemmar · Cellular Physiology and Biochemistry · 2013 · 47.3K citations · Full text