The detection of apple chlorotic leafspot virus by a modified procedure of enzyme‐linked immunosorbent assay (ELISA)

Abstract

SUMMARY The technique of enzyme‐linked immunosorbent assay (ELISA) was modified to enable detection of apple chlorotic leafspot virus (CLSV) both in herbaceous hosts and in several naturally infected fruiting and ornamental woody host species. Some of the characteristics of the modified method as used with different virus‐host combinations are described.