Analytical Letters · 1987 · 38 citations · 2 references
EngineeringChemistryDairy ScienceBiosensorsElectroanalytical MethodsElectrochemistryElectrochemical BiosensorsElectrochemical DetectionElectrochemical SensorsEnzymatic BiosensorsEnzyme-based BiosensorsMilk EnzymesEnzyme-based SensorsGlucose OxidaseHydrogen Peroxide BiosensorsLactose BiosensorsLactose DetectionLactose QuantificationPlatinum ElectrodesResponse Time
Abstract A hydrogen peroxide electrochemical sensor, coupled with two enzymes (ß -galactosidase and glucose oxidase) immobilized on the surface of the sensor covered with a cellulose acetate dialysis membrane (cut off 100), is proposed for the analysis of lactose in raw milk. The resulting hydrogen peroxide formation is measured with a platinum electrode polarized at +650 mV versus Ag/AgCl. The procedure developed is very simple and the short response time permits its use in assaying milk samples on dairy farms.
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