Tissue culture of <i>Aloe arborescens</i> Miller var. <i>natalensis</i> Berger

Abstract

Abstract We examined the culture conditions for callus induction in the tissue of Aloe arborescens . When incubated in the dark at 25°C, for about 30 days callus was induced. The frequency of callus formation was in the order of superior stalk &gt; middle stalk &gt; inferior stalk. No callus was formed in the leaf. When the Murashige–Skoog (MS) basal agar medium was supplemented with 3% sucrose, 0.1–0.5 μ M kinetin (a plant growth regulator) and 10–50 μ M alpha‐naphthalenacetic acid, callus formation occurred at an incidence of 20–52% (mean: 39%). When the MS basal agar medium was supplemented with 3% glucose and 10% Alpha Modification of Eagle's Medium instead of 3% sucrose, callus formation was promoted, but the protein and aloin concentrations, and carboxypeptidase activity in the Aloe callus decreased more than those observed in the sucrose‐containing medium.