Molecular cloning and expression of brain-derived neurotrophic factor

Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia

Randall K. Saiki, Stephen J. Scharf, Fred Faloona et al. · Science · 1985 · 9K citations

Medicine, Genetics, Molecular Genetics +17

High-efficiency transformation of mammalian cells by plasmid DNA.

C Chen, Hiroto Okayama · Molecular and Cellular Biology · 1987 · 5.3K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +13

Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.

Michael A. Frohman, Michael Dush, Gail R. Martin · Proceedings of the National Academy of Sciences · 1988 · 4.6K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +13

RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination

Hans Lehrach, Don J. Diamond, John M. Wozney et al. · Biochemistry · 1977 · 3.5K citations

Life Sciences, Biochemistry, Molecular Biology +8

A gas-liquid solid phase peptide and protein sequenator.

Rodney M. Hewick, Michael W. Hunkapiller, Leroy Hood et al. · Journal of Biological Chemistry · 1981 · 2.2K citations · Full text

Life Sciences, Biochemistry, Biotechnology +17