Probe-label-free electrochemical aptasensor based on methylene blue-anchored graphene oxide amplification

Abstract

Two novel probe-label-free electrochemical aptasensors based on methylene blue (MB)-anchored graphene oxide (GO) amplification were developed for thrombin (TB) and ATP detection, taking advantage of the specific binding affinity of the aptamer towards the target and the different affinities of GO for MB, ssDNA, dsDNA, and G-quadruplex.