Bioscience Biotechnology and Biochemistry · 1995 · 39 citations · 0 references
Life SciencesBiochemistryMicrobiologyBacterial Plant PathologyBacteriologyProteomicsAmino Acid ChemistryAmino Acid StructurePeptide AnalysisPeptide Bond ChemistryPeptide ChemistryPeptide IdentificationAmino AcidsHydroxy Fatty AcidsMass Spectrometry IdentificationPeptide Mass SpectrometryPeptide-mass FingerprintingPseudomonas SpeciesStructural Comparative Analysis
New syringopeptins SP(SC)-1 and -2 were isolated from culture filtrates of phytopathogenic bacterium strain SC1 of Pseudomonas syringae pv. syringae. These syringopeptins were composed of a beta-hydroxy fatty acid, a long sequence of aliphatic amino acids. and a lactone moiety of eight amino acids. The amino acid sequences were deduced from a comparison of their tandem mass sepctra with those of known syringopeptins SP-22a and SP-25a. SP(SC)-1 and SP(SC)-2 resembled SP-22a, but differed from the latter by 3 amino acids.