Scandinavian Journal of Immunology · 2003 · 32 citations · 39 references
Veterinary MedicineImmunologyInfectious DiseasesCellular ImmunologyImmunology Of Infectious DiseasesInflammationCytokine BiologyHost-parasite Immune InteractionsMacrophage BiologyMacrophage Responses To ParasitesPhagocyte BiologyProtozoan ImmunologyCell ActivationChemokine BiologyChemokine ModulationImmunopathology Of LeishmaniasisInflammatory ChemokinesLeishmaniasis PathogenesisLeishmaniasis PathologyReverse Transcription Polymerase Chain Reaction (Rt-pcr)
The expression of chemokines and chemokine receptors was studied in Leishmania donovani (LD)-infected human mononuclear phagocytes and the human monocytic cell line THP1. Our studies showed that LD infection caused the upregulation of three beta chemokines (macrophage inflammatory protein-1 alpha (MIP-1alpha), MIP-1beta and RANTES (regulated on activation normal T cell expressed and secreted)), one alpha chemokine (interleukin-8 (IL-8)) and the CC chemokine receptor 5 (CCR5) but not CCR1, as evident from reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. The CCR5 upregulation in human mononuclear phagocytes and THP1 cells was also evident by confocal microscopy. The possible association of such upregulation in relation to Leishmania and human immunodeficiency virus (HIV) coinfection was discussed.
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