A single‐step, sensitive flow cytofluorometric assay for the simultaneous assessment of membrane‐bound and ingested <i>Candida albicans</i> in phagocytosing neutrophils

Abstract

Our fluorescence flow cytometric method enables to simultaneously distinguish, within the leukocyte population, cell subsets with attached and ingested yeast particles. Its major features are: (1) accuracy, sensitivity and reproducibility; (2) no further sample manipulations after completion of phagocytosis; (3) possibility of counting free, attached and internalized yeast particles; and (4) use of a nontoxic reagent (TB).