HPTLC fingerprint profile of Bauhinia variegata Linn. leaves

Abstract

To develop the finger print of medicinally and economically important leaves of Bauhinia variegata Linn. Ethanol extract of the leaves were developed in the mobile phase of n-Hexane: Ethyl acetate: Formic acid: Acetic acid (70:30:1.0:1.0) using standard procedures and scanned under UV at 254 nm, 366nm and under visible light. The HPTLC fingerprinting of the ethanol extract has shown several peaks with different Rf values. 2.5 μL of ethanol extract showed 11 spots while 5 μL and 10 μL has shown 13 spots. 15μ L concentration gave 14 spots in the above said solvent system. This finger print would be helpful in the identification and authentication of this species.