Separation of the ginsenosides fraction obtained from the roots of<i>Panax quinquefolium</i>L. cultivated in Poland

Abstract

The objective of the work described in this paper was to investigate the separation of ginsenosides by TLC, HPTLC, and OPLC. The best separation of the ginsenosides was obtained by use of OPLC on silica gel 60F 254 with chloroform-methanol-ethyl acetate-water-hexane, 20 + 22 + 60 + 8 + 4 ( v/v ), as mobile phase. Results from TLC, HPTLC, and OPLC analysis of ginsenosides indicated that use of the different methods of development resulted in significant differences between the resolution of the compounds. Forced-flow OPLC is the most sensitive method for analysis of the ginsenosides.