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Acridine Orange/Ethidium Bromide (AO/EB) Staining to Detect Apoptosis
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2006
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EngineeringMicroscopyApoptosisCell DeathPathologyMolecular BiologyCell BiophysicsCellular PhysiologyOxidative StressBioanalysisAcridine Orange/ethidium BromideMolecular ImagingMicroscopy MethodsIn Vivo SystemBiomedical AnalysisCell BiologyShailaja KasibhatlaBiomolecular ScienceUltrastructureDavid L. SpectorCellular BiochemistryMedicineLaboratory Protocol
Shailaja Kasibhatla, Gustavo P. Amarante-Mendes, Deborah Finucane,Thomas Brunner, Ella Bossy-Wetzel and Douglas R. GreenThis protocol was adapted from “Apoptosis Assays,” Chapter 15, in Cells (eds. Spector etal.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1998. Thisthree-volume set is now out of print; however, some of the microscopy methods wererepublished in Basic Methods in Microscopy, by David L. Spector and Robert D.Goldman.