LARGE-SCALE BICORTICAL SKULL BONE REGENERATION USING EX VIVO REPLICATION-DEFECTIVE ADENOVIRAL-MEDIATED BONE MORPHOGENETIC PROTEIN—2 GENE—TRANSFERRED BONE MARROW STROMAL CELLS AND COMPOSITE BIOMATERIALS

Abstract

The Ad5 E1A-deleted AdV may be the optimal starting vector in ex vivo gene therapy for benign skeletal diseases. Additionally, the use of the gelatin/tricalcium phosphate ceramic/glutaraldehyde biopolymer with AdV BMP-2 gene transfer strongly enhances the bony healing of critical-size bicortical craniofacial defects. This method can be used by modifying the delivery of constructs to malunion treatment, in regional osteoporosis therapy, and spinal fusion.