PARTIAL PURIFICATION AND CHARACTERIZATION OF AN ACID RIBONUCLEASE IN BEEF BRAIN NUCLEI

Abstract

Abstract— In this report we describe the partial purification and characterization of an acid ribonuclease from beef brain nuclei (RNAase BN2). RNAase BN2 was purified approximately 85‐fold. The optimum pH is 6‐2 and the optimum temperature 55°C. The effect of ions on the RNAase BN2 and its K m were determined. RNAase BN2 is an endoribonuclease capable of hydrolysing polyA, polyU and polyC. Oligonucleotides produced by the hydrolysis of polyA by the RNAase BN2 have a monophosphate group at the 3′ position.