Nucleic Acids Research · 1983 · 101 citations · 22 references
Life SciencesMicrobiologyMolecular BiologyGene ExpressionGenetic EngineeringMolecular BiotechnologyBacteriophage VectorsPlasmid BiologyPlasmid GeneticsPromoter DesignProtein SynthesisRecombinant Plasmid ConstructionEscherichia ColiInitiator ElementsInterferon Gene InductionInterferon ProductionPhage PromotersPlasmid Vector ConstructionPlasmid Vector DesignPlasmid Vector Engineering
We have obtained high level synthesis in Escherichia coli of mature human fibroblast interferon using a plasmid vector that was designed to allow easy coupling of a DNA coding region to the initiator AUG of the replicase gene of the RNA phage MS2 cloned downstream of phage lambda's leftward promoter. The activity of the promoter can be regulated by temperature. Induced cells accumulated the interferon up to 4% of the total cellular protein. The biological activity of the product amounted to 4 X 10(9) international units per litre of culture. The synthesis of human fibroblast interferon was shown to drastically inhibit the growth rate of the bacterial host.
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