Obtaining of the essential oil of Syzygium aromaticum, identification of eugenol and its effect on Streptococcus mutans.

Abstract

Dental caries is a disease of the oral cavity in humans. Currently seeking active principles of plants with antimicrobial effect represents a promise in Dental Therapy. This study evaluated the activity of the essential oil of Syzygium aromaticum (clove) with emphasis on its antimicrobial property. The oil was obtained by hydrodistillation, characterized by thin layer chromatography and chemical tests. The major compound was identified in the oil obtained from the flower buds and their antibacterial activity was evaluated against planktonic cells Streptcoccus mutans ATCC (700,611) performed serial dilutions; from 15 to 1000μg / mL and compared with 0.12% chlorhexidine and dimethylsulfoxide also the MIC was determined. Subsequently, the UFC was analyzed and compared with the Test CMR® Ivoclar Vivadent. The efficiency of oil extraction was 2.20%. CCD technique by the disclosed UV fraction corresponding to eugenol was identified. He had a good response to flavonoids and triterpenes. He showed greater antimicrobial activity at concentrations of 1000, 500 and 250 μg/mL. The MIC and MBC of the oil was 125 and 250 µg/mL respectively. The presence of eugenol as an active ingredient in the oil obtained was checked. Projection currently use of plant extracts has led to the evaluation of biocompatible for use in the formulations for oral hygiene products applied to the prevention or treatment of oral diseases, effective and alternative antibacterial agents