Development of microsatellite primers and two multiplex polymerase chain reactions for the common elder (<i>Sambucus nigra</i>)

Abstract

Abstract Primer pairs were developed for eight microsatellite loci isolated from a genomic DNA library of Sambucus nigra ‘Black Beauty’. In a survey of 21 accessions of S. nigra, all revealed polymorphism and the number of alleles per locus ranged from three to 18. Two multiplex polymerase chain reactions (PCRs) were optimized and these discriminated all the accessions tested providing a cost‐effective genotyping solution for the species. Six primer pairs (EMSn002, 003, 010, 016, 017, and 023) were informative in two Sambucus canadensis accessions.