The Expression of Porcine Adiponectin and Stearoyl Coenzyme a Desaturase Genes in Differentiating Adipocytes

Abstract

For cloning of gene fragments and tissue distribution studies, three crossbred pigs (Sus domesticus; sows were predominantly Landrace-Yorkshire crossbreds mated to a Duroc boar) at age 8 to 9 wk old were killed, with an electrocution combined with exsanguinations 2 h after feeding. The protocol was approved by the Experimental Animal Management and Use Committee at National Taiwan University. Heart, kidney cortex, liver, longissimus muscle, and subcutaneous adipose tissue were quickly dissected and frozen in liquid nitrogen with storage at -70C until RNA extraction.