Evidence for the existence of a family of bacterial nitroreductases capable of activating nitrated polycyclics to mutagens

Abstract

A derivative of Salmonella typhimurium TA98 which does not respond to the potent mutagenicity of 1,8-dinitropyrene is described. This novel strain also shows a lack of response to the mutagenic action of 1,3-dinitropyrene and a greatly reduced response to 1,6-dinitropyrene and 1-nitropyrene. The responses to 1,3,6-trinitropyrene and 1,3,6,8-tetranitropyrene are affected to a much lesser extent. This strain (TA98/1,8DNP6) is fully sensitive to the mutagenicity of 4-nitroquinoline-1-oxide, niridazole, nitroacridine, and nonnitrated frameshift mutagens. This strain appears to be deficient in a nitroreductase which reduces nitrated pyrenes and possibly other nitrated polycyclic aromatic hydrocarbons to corresponding hydroxylamines, the penultimate mutagens.