Concepedia

Cloning and Expression of DNA Coding for the Major House Dust Mite Allergen Der p 1 in Escherichia coli

Wayne R. Thomas, G.A. Stewart, Richard J. Simpson, K.Y. Chua, T.M. Plozza, R.J. Dilworth, A. Nisbet, Kati Turner

International Archives of Allergy and Applied Immunology · 2009 · 119 citations · 0 references

Concepts

Life SciencesImmunologyMolecular BiologyAllergyGene ExpressionMicrobial GeneticsAllergen RecognitionAllergic Disease PathologyAllergen PurificationCdna CloningCdna Library ConstructionCdna SynthesisDust Mite Allergen IdentificationExpression Library ConstructionHouse Dust Mite Allergens

Abstract

A cDNA clone coding for the major house dust mite allergen Der p 1 was isolated from a lambda gt 11 library. Its sequence correlates with known amino acid sequences of Der p 1 and it produces a fusion protein which reacts with rabbit anti-Der p 1 antiserum.